Maturation of porcine oocytes by EGF treatment via activation of AKT signaling.

(A) Western blot analysis (p-AKT, AKT, and a-tubulin) was performed using oocytes from class I and class II COCs for each indicated group. (B) Effect of EGF treatment on the blastocyst formation rate of the indicated classes. Representative photographs of blastocysts (left) and blastocyst development rate of the indicated classes (right). Bar = 50 μm. The data are from three independent experiments, and the values represent the means ± SEM (*P P P < 0.05). (G) Nuclear maturation rate of mature oocytes from the indicated classes.