Additional file 12: of Factors driving effective population size and pan-genome evolution in bacteria

Figure S10. Association between Ne and pan-genome size, adjusted for sample size (Spearman’s rho = 0.48, P < 10− 8, PIC correction). Ne was estimated from dS/dN ratios (Fig. 1). Pan-genome sizes were corrected for sample size by analyzing the same number of genomes for each species while maximizing the divergence rate of the core genome. Using a recursive approach, the pair of the most similar genomes for a species was identified, and one of the two genomes was randomly discarded. This process was repeated until each species was down-sampled to 13 genomes. The pan-genome was then re-built for each species as described above. (PDF 154 kb