Echocardiographic analysis of multiple morphological and functional parameters in IR/LepA and IR/saline treated mice.

(A-E): Echocardiography was performed at baseline, 24 hours and 30 days postoperatively. A: Increase in left ventricle (LV) end-diastolic diameter (EDD) and end-systolic diameter (ESD); B. Increase in LV volume at diastole and systole; C. Increase in endocardial (Endo) area in diastole and systole; D. Endo area change, Endo fractional area change (FAC)%, and LV fractional shortening (FS)%; E. Ejection fraction (EF)% on day1 and day 30. Values show average percent of change ± SE from baseline measurements. In all figures orange bar depicts IR/LepA treated mice, and blue bars IR/Saline treated mice. * p<0.05, ** p<0.01 by two-tailed unpaired student’s t-test between the two groups. Error bars indicate standard error. (data collected from 14 IR/saline and 16 IR/LepA treated mice).