Rhodomyrtone suppressed the TNF-α induced phosphorylation of MAPKs and NF-κBp65 in primary human keratinocytes.

Keratinocytes were pre-incubated with 600 ng/ ml rhodomyrtone for 24 h and stimulated with TNF-α for 0, 10, 20, 30, and 60 min. The ratio of p-ERK/ERK (a), p-p38/p38 (b), p-JNK/JNK (c), and p-p65/p65 (d) was analyzed by western blot analysis, and protein expression was quantitated using Image Studio Lite v5.2.Ink software and normalized to β-actin. Values are mean ± SD of results from three independent experiments in triplicate. (*pppp<0.0001, as compared with TNF-α at each time point).