Cofilin-actin rod formation after transient focal ischemia.

a. Lesioned area as identified by NeuN immunostaining in brain fixed 24 hours after a 30-minute MCA occlusion. Blue line marks the approximate medial edge of the lesion, and blue box shows the area photographed on each section after cofilin-1 immunostaining. b. Immunostaining for cofilin-1 (red) in the ischemic cortex at designated time points after reperfusion. Cell nuclei are stained blue. c. Graph quantifies area of cofilin staining at each time point, expressed as percent of the image area (n = 4–5; *p < 0.05 v. sham).