Introducing an expanded CAG tract into the huntingtin gene causes a wide spectrum of ultrastructural defects in cultured human cells - Fig 3

Assessment of the proliferation rate (a) and viability (b) of three mutant clones (8D, 8H, and 6H) compared to the original HEK293 cell line. (a) The proliferation assay of mutant clones was conducted by real-time cell analysis. The negative peak in the plot (a) is the consequence of medium replacement (approximately 48 h after the start of the experiment). (b) Apoptosis intensity in mutant cell clones was estimated by an annexin V/propidium iodide cell viability assay. Data scatter of early apoptotic cells (annexin V positive / PI negative) is presented. The bars show standard deviation, n = 3.