Mass spectrometry-based proteomic analysis of AuP and healthy cardiomyocytes.

[A] Unsupervised hierarchical clustering of 301 proteins with significantly different expression in healthy (clone 24.5) and AuP cardiomyocytes (FDR 0.05, S0 0.5). Technical triplicates are shown for each sample. Healthy 1 and 2 indicate two unrelated control individuals. [B] Bar graphs showing the fold increase of the top three annotation enrichments in the cluster of proteins that are upregulated (red bars) and downregulated (green bars) in the AuP cardiomyocytes. The corresponding p value is reported next to each bar. [C] Volcano plot of statistical significance against fold-change (FDR 0.05, S0 0.5), highlighting the proteins with higher expression in control (black) and AuP (blue), respectively. [D] Bar plots depicting the log2 fold change expression of selected cardiac Ca2+ handling proteins that are differentially expressed between AuP and both healthy control cardiomyocytes.