Comparison of binding of various anti-HIV bnAbs to yeast surface displayed HIV envelope from 4 viral strains.

The “m” preceding the HIV strain name indicates the modified dsm version. The concentrations of the various antibodies are as follows: anti-V5, 20 nM; anti-gp120 polyclonal, 60 nM; all others, 100 nM. Where indicated, soluble CD4 (sCD4) was used at a concentration of 100 nM. If the mean fluorescence after subtraction of the mean fluorescence of secondary plus cells was negative, it was assigned a value of zero. The color scale is non-linear. Secondary antibody incubations were performed at 4°C and cells were maintained on ice prior to flow cytometry. Further details of these binding determinations are tabulated in S1 and S2 Tables.