Additional file 1: of Genotoxic and epigenotoxic effects in mice exposed to concentrated ambient fine particulate matter (PM2.5) from São Paulo city, Brazil

Table S1. Reagents used in the study. Table S2. Parameters used in the ESI-MS/MS equipment for detection of the lesions and epigenetic marks in DNA. Figure S1. System of two columns used for 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) analyses. A) Configuration used in the first 16 min and from 32 to 46 min of the chromatography; B) Configuration used in the interval 16 – 32 min, allowing further separation and peak narrowing in column B prior to elution to the ESI source of the mass spectrometer. Figure S2. Calibration curves obtained by HPLC-ESI-MS/MS for quantification of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo), 1,N2-etheno-2′-deoxyguanosine (1,N2-εdGuo), 1,N6-etheno-2′-deoxyadenosine (1,N6-εdAdo), 2′-deoxycytidine (dCyd), 5-methyl-2′-deoxycytidine (5-mC), and 5-hydroxymethyl-2′-deoxycytidine (5-mC, lower range). (DOCX 738 kb