Icosahedral reconstruction of the authentic <i>Mr</i>NV virion at 6.6 Å resolution.

CryoEM was used to calculate an intermediate-resolution 3D reconstruction of the authentic MrNV virion, purified from infected M. rosenbergii post-larvae (a). This revealed a structure that, at this resolution, was indistinguishable from the VLP reconstruction. As in our VLP reconstruction, the virion map reveals the presence of an internal dodecahedral density that we attribute to viral genome (b). CryoEM, cryogenic electron microscopy; MrNV, M. rosenbergii nodavirus; 3D, three-dimensional; VLP, virus-like particle.