Plasma Na⁺, Cl^-, K⁺, Ca²⁺concentrations, hematocrit, and strong ion ratio (SIR; Na⁺:Cl^-) and muscle water content (MWC) and muscle Na⁺ and K⁺ concentrations, and muscle Na⁺/K⁺ ratio of <i>P</i>. <i>lineatus</i> acclimated to [brackish water (BW), brackish water ligated (BW-L) 3‰, seawater (SW-control), seawater ligated (SW-L) 34‰] salinity).

Data are means ± S.D. (n = 7–9). Means within a given parameter across treatment groups which do not share the same letter are significantly different from one another (one-way ANOVA, SNK).