Homologs of RIP2CARD.

At top, the aminoacid sequences of homologous to the rat RIP2 RIPK2 proteins are aligned with RIP2CARD sequence. At bottom,CARD domains of the proteins with other functions are aligned based on the 3D structure homology, according to the PDBefold server, PDB IDs are indicated. Conservative residues are highlighted with respect to their physical properties (yellow-hydrophobic, blue—positively charged, red—negatively charged, green—polar or neutral). Lines indicate the salt bridges, observed in RIP2CARD structure. Red, magenta, blue and cyan circles denote the residues which were shown important for the human RIP2-p75 [13], NOD2/RIP2, RIP2/NOD1/NOD2 and exclusively RIP2/NOD1 interactions [15]. Brown circles denote the residues, important for the unique packing of the RIP2CARD C-terminal residues.