Evaluation of mAb purity and surface binding to HER2 receptor.
- Jianfa Ou (5883983)
- Yingnan Si (5883986)
- KahYong Goh (5883989)
- Norio Yasui (1479013)
- Yichen Guo (4402723)
- Jiajia Song (1412371)
- Lizhong Wang (25250)
- Renata Jaskula-Sztul (5883992)
- Jinda Fan (1484344)
- Lufang Zhou (254131)
- Runhua Liu (2177686)
- Xiaoguang Liu (109982)
Publication date
October 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
(a) SDS-PAGE gel. M: marker, 1: anti-HER2 mAb purified by small-scale protein A purification kit, 2: anti-HER2 mAb purified by large-scale NGC system, 3: FDA approved Trastuzumab. Protein samples were loaded with 1 μg/well. (b) Flow cytometry analysis of receptor binding of purified anti-HER2 mAb and Trastuzumab to HER2+ BT474 cell line and negative control MDA-MB-468 cell line. Staining conditions: Alexa Fluor (AF) 488-labeled mAb was incubated with cells at 1 μg/million cells on ice for 30 min.</p
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