Additional file 2: of Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid

Figure S2. Confirmation of mTn5 transposition events in A. baylyi and in P. aeruginosa. (a) Colony restreaking assay of A. baylyi. 100 SucRKanR colonies of A. baylyi were restreaked on LB + Kan and LB + Cam plates, and all were found to be KanRCamS. 50 are shown here. (b) Colony PCR of 10 restreaked A. baylyi clones with the indicated primers. All were mTn5-positive and plasmid-negative. (c) Colony restreaking assay of P. aeruginosa. 100 SucRKanR colonies of P. aeruginosa were restreaked on LB + Kan and LB + Cam plates, and all were found to be KanRCamS. 50 are shown here. (d) Colony PCR of ten restreaked P. aeruginosa clones with primers indicated in the diagram. All were mTn5-positive and plasmid-negative. (TIF 6384 kb