Hematoxylin-eosin staining of UUO kidneys shows improved histology with PEG-FUD treatment.

Representative images from the central cortex of 4 μm kidney sections stained with H&E. UUO and contralateral kidneys were stained simultaneously. Except for the contralateral kidney of saline treated mice, all other images are from UUO kidneys for comparison of treatment with saline, PEG, FUD, PEG-FUD and PEG-mFUD. Eosin staining was more prominent in the saline treated contralateral kidney, was associated with intact proximal tubules (arrowheads) and with defined glomeruli containing red blood cells (double asterisks). Hematoxilin staining associated with infiltrating cells, interstitial cell proliferation (arrows) and less defined glomeruli lacking red blood cells (single asterisk) were more prominent in the saline, PEG or PEG-mFUD-treated UUO kidneys. Diminished hematoxylin, increased eosin staining associated with tubular structures, and vascularized glomeruli similar to the contralateral kidney, were more apparent in the FUD and, to a greater extent, the PEG-FUD-treated kidneys, suggesting improvement in tubular and glomerular health. Bar = 100 μm.