Additional file 2: of Preconditioning of bone marrow-derived mesenchymal stem cells highly strengthens their potential to promote IL-6-dependent M2b polarization

Figure S2. Characterization of M0, M1- and M2a-like macrophages. (a) CD11b and F4/80 expression on bone marrow-derived differentiated M0 macrophages. M0 macrophages were further polarized in vitro to M1-like and M2a-like cells by treatment with 20 ng/ml IFN-ɣ and 100 ng/ml LPS (M1) or 20 ng/ml IL-4 (M2), respectively, for 24 h. (b) Gene expression of iNOS and Arg I was determined by real-time PCR. n = 3. (c) TNF-α and IL-6 levels in culture supernatants were quantified by Elisa. n = 5, *p < 0.05, **p < 0.001. (TIF 1040 kb