MOESM1 of An oleaginous yeast platform for renewable 1-butanol synthesis based on a heterologous CoA-dependent pathway and an endogenous pathway

Additional file 1: Table S1. Primers used for the construction of the heterologous CoA-dependent pathway. Figure S1. GC/MS detection of 1-butanol production in two Y. lipolytica engineered strains carrying the chromosomally integrated plasmid pYLEX1-All-GPD or pYLEX1-All-MDH. Figure S2. Map of the plasmids pYLEX1, pYLEX1-2 and pYLEX1-3. Figure S3. Map of the plasmid pYLEX1-A1HB. Figure S4. Map of the plasmid pYLEX1-A2CR. Figure S5. Map of the plasmid pYLEX1-A2CREuET. Figure S6. Restriction map of pYLEX1-NEW. Plasmid pYLEX1-NEW with an improved version of MCS was constructed by adding XbaI, AvrII, MfeI and MluI sites into the BamHI site of pYLEX1. Plasmid pYLEX1-NEW contains two BamHI sites. Details on DNA sequence alignment and additional Materials and Methods are also provided as additional information