ELISAs to detect human MDP1, CFP10, ESTA6, Ag85, and PPD-specific IgG antibodies.

The results of ELISAs performed to detect IgG antibodies that recognize recombinant proteins and PPD in human blood samples. The tested sera in A-I were from 12 past tuberculosis (Past TB) patients, 8 active tuberculosis patients (Active TB), and 10 healthy control (Healthy) individuals (also for J). Additionally, sera derived from 23 individuals with past tuberculosis were tested in Fig. 4J. (A) IgG responses to AC-rFull-MDP1, which was purified by acid extraction. (B–C) IgG responses to rFull-MDP1, purified by the refined purification method, was immobilized in buffer containing 150 mM NaCl (B) or 2 M NaCl (C). (D–E) IgG responses to rN-MDP1 immobilized in 150 mM NaCl (D) and 2 M NaCl (E). (F–I) IgG responses to CFP10 (F), ESAT6 (G), Ag85 (H), and PPD (I). (J) IgG responses to rFull-MDP1 of the 10 healthy control and those to rFull-MDP1, rN-MDP1, and rC-MDP1 of 23 other past TB individuals. *, p<0.05. **p<0.01.