High content lipid quantitation in PH5CH8 liver cells.

Negative control cells stimulated for de novo lipid synthesis showing nuclei (gray) and lipids (green) (A) and lipid quantitation of negative control using high-content image analysis resulting in a high lipid score, normalized to 0% effect (C) Positive control—unstimulated cells representing the normal (basal) lipid content with a low lipid score and normalized to 100% effect (B). Representative images from high throughput screening showing negative (D) and positive (E) control wells and stimulated cells treated with 1 μM niclosamide (F). Dose response analysis of test compounds Triacsin C (G) and niclosamide (H) shown in blue. Green and red lines represent the mean and standard error of stimulated and unstimulated cells.