Andrographolide with radiation enhanced downregulation of radiation-induced MMP-2 leading to the suppression of ERK signaling.

The cells were treated with 10 uM andrographolide with/without 2Gy or 4 Gy radiation for 24 hours. The figure displays typical data of MMP-2, phosphor-ERK1/2, total-ERK1/2 and NF-κB activity by Western blotting. Relative band intensities were analyzed by the Image J software. β-actin was used as an internal control for normalization. The numbers beneath the blots indicate the relative expression of each band when compared to the respective untreated control.