Analysis of NO in ZIKV-infected microglial cells.

CHME5 cells were exposed to ZIKV (MOI 1) and expression of iNOS and eNOS was determined by real-time RT-PCR (A). Results are expressed as the fold induction of transcripts in ZIKV-infected cells, relative to those in mock-infected cells. Data are representative of four independent experiments, each performed in duplicate (error bars represent SEM). The Wilcoxon-Mann-Whitney test was used to analyze the differences between sets of data. p-values of p-values of <0.05 were considered significant (*).