Patient-derived xenografts from cervical dysplasia and normal cervical tissue.

A) Two representative examples of the cystic structures formed from cervical dysplasia xenografts. H&E (first column) and immunohistochemical staining for human nuclei antibody (brown nuclei), human p16INK4a (brown nuclear staining), HPV (brown cytoplasmic staining), Insets, IgG isotype negative controls. Scale bars; 50 μm B) normal cervical tissue xenografts. Two representative examples of normal cervix after 6 months under the renal capsule of NSG mice, showing (i) cervical stromal tissue and (ii) cervical squamous epithelium. H&E, anti-human nuclear (brown nuclei), human p16INK4a (brown nuclei) and HPV immunostaining of harvested xenografts. Note that unexpectedly, HPV is present in the second sample. Arrows indicate examples of positively stained nuclei. Dotted line; border with mouse kidney. K marks kidney. Insets show IgG isotype controls. Scale bars 10 μm.