Adoptively transferred bone marrow monocytes differentiate into diverse cell populations in tissues of <i>M</i>.<i>tuberculosis</i>-infected mice.

Bone marrow monocytes from CD45.1+ mice were depleted of stem cells and transferred to M. tuberculosis-infected CD45.2+ mice, 4 or 8 weeks after infection. CD45.1+ cells were identified by flow cytometry at multiple time points after transfer; cells in the lung vasculature were distinguished from cells in the lung parenchyma by the presence of labeling with a pan-CD45 antibody injected intravenously immediately before euthanasia. A) Subset frequency, as the proportion of the total CD45.1+ cells recovered; B) total subset numbers of CD45.1+ donor cells in blood, lung vasculature, lung parenchyma, lung-draining mediastinal lymph node (MLN) and total pooled peripheral LN (pLN). Data are from single experiments with 5 mice per time point. Proportion of transferred cells are means, and absolute cell numbers are means ± SEM.