Recently proliferated mononuclear cells enter lung granulomas and become infected by <i>M</i>. <i>tuberculosis</i>.

Mice infected with fluorescent protein-expressing M. tuberculosis were injected with EdU and its incorporation by dividing mononuclear cells evaluated by fluorescence microscopy or flow cytometry. Representative immunofluorescent staining of A) lung granulomas or B) CD11b+ lesions in the MLN of mice infected with GFP-expressing M. tuberculosis, 3 or 2 days after EdU pulse, respectively. Arrows indicate EdU+ nuclei adjacent to GFP-expressing M. tuberculosis bacilli. Images are representative of 1–2 mice per time point. C) Flow cytometric analysis of the frequency of EdU+ phagocyte subsets that were infected with Rv-mCherry at multiple time points following EdU pulse 4 weeks post infection. D) Flow cytometric analysis of total number of Rv+ EdU+ phagocyte subsets at multiple time points following EdU pulse 4 weeks post infection. E) Flow cytometric analysis of the frequency of Rv-mCherry-infected professional phagocytes that were EdU+ at multiple time points following EdU pulse 4 weeks post infection. Data are presented as means ± SEM of 4–5 mice per time point from a single infection for each phase. Statistics are 2-way ANOVA with Sidak’s multiple comparisons tests. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.