MOESM1 of Effect of lignin fractions isolated from different biomass sources on cellulose oxidation by fungal lytic polysaccharide monooxygenases

Additional file 1: Figure S1. Cyclic voltammograms of (A) HT-A-WS, (B) SE-S, (C) SE-B, (D) OS-S, (E) OS-B, (F) OS-A-S, (G) OS-A-B, (H) OS-S-LF, (I) OS-B-LF, (J) OS-A-S-LF and (K) OS-A-B-LF immobilized on a glassy carbon electrode with the use of Nafion in 100 mM tartrate buffer pH 5.0, at 50 oC between -200 and 600 mV vs Ag/AgCl for a scan rate of 5 mV/s. Figure S2. FTacV 1st to 6th Harmonic of HT-A-WS lignin immobilized on a glassy carbon electrode with the use of Nafion for a v = 50 mV/s, A = 110 mV, f = 12.3 Hz in de-aerated 100 mM tartrate buffer pH 5.0 at 50ÎżC. Figure S3. The products of MtLPMO9 action on PASC after addition of ascorbic acid 1 mM or lignin isolated from pretreated materials 10 mg/mL. Products at 5-13 min correspond to neutral sugars, 13-19 min to C1 oxidized sugars, 19-25min to C4 oxidized sugars and 25-30 min to mixed C1/C4 oxidized sugars. Peaks at 12.3, 21.8 and 31.6 min are assigned to ascorbic acid. Figure S4. The products of PcLPMO9D action on PASC after addition of ascorbic acid 1 mM or lignin isolated from pretreated materials 10 mg/mL. Products at 5-13 min correspond to neutral sugars and 13-19 min to C1 oxidized sugars. Figure S5. The products of NcLPMO9C action on PASC after addition of ascorbic acid 1 mM or lignin isolated from pretreated materials 10 mg/mL. Products at 5-13 min correspond to neutral sugars and 19-25 min to C4 oxidized sugars. Peaks at 12.3, 21.8 and 31.6 min are assigned to ascorbic acid. Figure S6. GPC chromatograms for HT-A-WS lignin before and after 24h-incubation with buffer phosphate-citrate 100 mM pH 5.0 and buffer/MtLPMO9. Table S1. Estimated aliphatic and aromatic groups content (mmol/g) of HT-A-WS lignin before and after 24h-incubation with buffer phosphate-citrate 100 mM pH 5.0 and buffer/MtLPMO9, as evaluated from 31P NMR. Table S2. Identified extractives from HT-A-WS lignin in different solvents