Adjuvantation of ChAdOx1 RabG and ChAdOx2 RabG.

Panel A shows effect upon ELISA-measured antibody responses of addition of Addavax to ChAdOx1 RabG. p = 0.02 for effect of adjuvant by 2-way ANOVA performed upon log10-transformed data. Panel B shows effect upon ELISA-measured antibody responses of addition of Addavax to ChAdOx2 RabG. Data for mice not receiving the adjuvant is as shown for ChAdOx2 in Fig 3A. p = 0.03 for effect of adjuvant by 2-way ANOVA performed upon log10-transformed data. Panel C shows effect upon ELISA-measured antibody responses of addition of SWE to ChAdOx2 RabG. Data shown combines results from three mouse cohorts: cohort A at doses 5x103, 5x104 and 5x105 IU; cohort B at doses 5x103 and 5x104 IU; cohort C at doses 1x106 to 1x108 IU. Each experiment included 6 mice at each dose level, hence 12 individual results are shown for doses 5x103 and 5x104 IU. Stars indicate significant effect of adjuvant at a single dose level, pooling all data (p = 0.05 at dose = 5x103 IU, p = 0.01 at dose = 5x104 IU, both by two-tailed Mann-Whitney test). 2-way ANOVA was not performed across the full dose-range in view of the lack of adjuvant effect at high dose (i.e. a dose-adjuvant statistical interaction). Panel D shows the data from all mice receiving the 5x103 and 5x104 IU doses in cohorts A and B. This is the same data shown in panel C, but separating the two cohorts to show consistency of effect across experiments. P = 0.0002 for effect of adjuvant by 3-way ANOVA performed upon log10-transformed data, with P = 0.001 for effect of dose and P = 0.0006 for effect of experiment; in keeping with the consistent trend of effect of adjuvant between doses and experiments, no statistical interaction between parameters was observed. Throughout, points indicate results from individual mice; lines indicate group medians. All results shown are from serum samples collected 4 weeks after immunization. Units are arbitrary.