<i>In vivo</i> experimental validation of cell cycle-related biological process predictions.

Phenotypic validation of cell cycle-related predictions, performed on drug-hypersensitive yeast treated with solvent control (DMSO) or compounds predicted to perturb the cell cycle. Out of 17 compounds predicted to arrest cells in G2/M phase, data are shown for the 6 that exhibited increases in the relevant phenotypes in any and all assays. Data for NPD7834 are also shown. (A) Differential interference contrast microscopy (DIC) and fluorescence upon DAPI staining showing bud size and DNA localization, respectively, after compound treatment. The scale bar represents a distance of 5 μm. (B) FACS analysis of cell populations in different cell cycle phases at 0, 2, and 4 hours after compound treatment. The green curve overlay represents the estimated cell population in G1, S and G2/M phases. (C) Budding index percentages induced by treatment with compound or solvent control.