IL-4Rα-mediated signaling on Foxp3⁺ Treg cell is required for eTreg cell expansion, recruitment, and sustainability during schistosomiasis.

(A) Representative flow cytometry of cTreg cells and eTreg cells from the hLN of mice infected with Sm for 8 wk. (B) Frequency of central CD4+ Foxp3+ Treg cells (left) and (C) effector CD4+ Foxp3+ Treg cells from (A). (D) Representative histogram of CXCR3 expression by effector CD4+ Foxp3+ T cells in the hLN 8 wk post infection with the mean values summarized in (E). (F) Representative histogram of GATA3 expression by CD4+ Foxp3+ T cells in the liver 8 wk post infection with the mean values summarized in (G). (H) Frequency of CD4+ Foxp3+ Helios+ Treg cells in the liver of Sm-infected mice. (I) Frequency of CD4+ Foxp3+ Helios− Treg cells in the liver of Sm-infected mice. (J) Representative histogram of Ki-67 expression by CD4+ Foxp3+ T cells in the liver 8 wk post infection with the mean values summarized in (K). (L) Representative histogram of Bcl-2 expression by CD4+ Foxp3+ T cells in the liver 8 wk post infection with the mean values summarized in (M). (N) Representative histogram of caspase-3 expression by CD4+ Foxp3+ T cells in the liver 8 wk post infection with the mean values summarized in (O). (P) Representative histogram of fixable dye in CD4+ Foxp3+ T cells in the liver 8 wk post infection with the mean values summarized in (Q). Results pooled from two independent experiments with 3–4 mice/group. Data are expressed as mean ± S.E.M. NS, P > 0.05; * P P P t test. Underlying data can be found in S1 Data. Bcl-2, B cell lymphoma 2; CD44, cluster of differentiation 44; CD62L, L-selectin; cTreg, central regulatory T; CXCR3, C-X-C motif chemokine receptor 3; eTreg, effector regulatory T; Foxp3, forkhead box P3; GATA3, GATA binding protein 3; GMFI, geometric mean fluorescence intensity; hLN, hepatic lymph node; IL-4Rα, interleukin-4 receptor alpha; NS, not significant; Sm, S. mansoni; Treg, regulatory T.