Schematic approach to define the threshold window for segmentation of ‘lesion area’ and the ventricular space.

MR images corresponding to a TBI mouse at 1, 7, and 14 days post-TBI are shown. The schematic approach to define the threshold window for segmentation of ‘lesion area’ (RED outline) and the ventricular space (BLUE outline) is depicted with the first column showing the MR images at different time points in normal gray scale with contrast optimized for whole brain visualization. The center column depicts the corresponding images in an adjusted threshold scale (S.I.~100–500 a.u.) that facilitates visualization of both lesioned area and ventricular space. The last column superimposes the segmentation of regions of interest with RED outlining the lesion area and BLUE outlining the ventricular space. The progressive ventricular enlargement and corresponding reduction of the lesion area are clearly evident and are consistent with loss of brain tissue.