Primary cell isolation light microscopy and immunofluorescence characterisation.

Light microscopy images showing typical morphologies for (A) isolated ECs and (B) SMCs. (C) ECs positively stained for the marker Von Willebrand factor (green). (D) Smooth muscle cells positively stained for alpha smooth muscle actin (red). (E) Negative control, ECs showing minimal staining for alpha smooth muscle actin (red). (F) Negative control, SMCs showing minimal staining for Von Willebrand factor (green). Images were taken of cells at passage 2. White scale bars are 100μm, all nuclei stained with DAPI (blue).