Additional file 7: of Sexually dimorphic metabolic responses mediated by CRF2 receptor during nutritional stress in mice

Male mice have increased plasma lipid levels. Column bar graphs showing plasma lipid profiles in male and female mice. Plasma cholesterol, HDL, triglycerides, and LDL levels were determined. (a) HFD-fed male WT mice had 37.7%, Crhr2+/− mice had 50.3%, and Crhr2−/− mice had 40.9% higher blood cholesterol levels vs. chow diet. (b) In female WT, Crhr2+/−, and Crhr2−/− mice, HFD consumption resulted in smaller, non-significant increases in blood cholesterol levels vs. chow. (c) HFD-fed male WT mice had 36.1% higher and Crhr2+/− had 38.3% higher HDL levels vs. chow. (d) In female mice, HDL levels did not differ between HFD vs. chow. (e) HFD-fed male Crhr2−/− mice had ~ 53.0% higher calculated LDL levels vs. chow-fed Crhr2−/− and HFD-fed WT mice, whereas Crhr2+/− had 68.0% higher LDL vs. chow. (f) In female mice, diet did not change LDL levels. (g) In male mice, diet did not change triglycerides levels. (h) Female Crhr2+/− mice had significantly elevated triglyceride levels on both chow and HFD compared with WT and Crhr2−/− female mice on chow and on HFD vs. WT chow. n = 8/group/sex. 3-Way ANOVA and post hoc Tukey’s multiple comparisons. (DOCX 524 kb