β-oxidation of fatty acids is upregulated in Dahomey larvae fed the 1:16 P:C diet.

(A) Triglyceride levels were higher in Dahomey larvae fed the control diet. When Etomoxir (Eto) was added to the control diet, triglyceride levels increased, and differences between the mitotypes was lost (n = 14 rep/mitotype/treatment with 6 failed reactions). (B) Expression of eloF) and bmm) were higher in Dahomey larvae fed the control diet. Differences were lost when Etomoxir (Eto) was added to the control diet (n = 6 rep/mitotype). (C) β-oxidation activity was highest in Dahomey larvae (n = 10 biological rep/mitotype–with one outlier removed). (D) Acetyl-coA enzyme activity in the cytosol and extracted mitochondria was higher in Dahomey larvae. (n = 9 biological rep/mitotype–with four outliers removed from the cytosol data). (E). NAD+/NADH ratio was higher in Dahomey larvae (n = 7 rep/mitotype). (F) Starvation survival was greatest in Dahomey larvae (n = 56 for Alstonville and 91 for Dahomey). Bars (mean ± s.e.m). p< 0.05 and p< 0.01, as calculated by t-tests (see text).