Primers and probe sequences, estimated annealing temperatures (Tm), and optimal primer concentrations of the eDNA assay for bull trout ITSI.
- Joseph C. Dysthe (3095415)
- Thomas W. Franklin (5938049)
- Kevin S. McKelvey (394722)
- Michael K. Young (394723)
- Michael K. Schwartz (108368)
Publication date
November 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
Optimal primer concentrations refer to the lowest concentration of primers resulting in the earliest Ct while maintaining a high end-point fluorescence. The assay amplifies a 172-nucleotide fragment of the bull trout ITSI gene.</p
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