Longitudinal EEG and EMG recordings in the neonatal mouse.

(A) Protocol summarizing the timing of the longitudinal recording sessions in individual mice. (B) After electrode placement surgery at P7 or P8, the attached electrode apparatus is of minimal size and the exposed pin header allows reversible connections for recording EEG and EMG while permitting pup feeding and group nesting. (C) Weight gain in operated and naïve mice. *p(D) Histological assessment of electrode placement and injury during EEG recording of the neonatal mouse. Top panels: Cresyl violet stained coronal sections display limited injury to underlying layer I/II cortex of naive or EEG recorded (Rec) mice caused by surgery or recoding paradigm. Bottom panels: GFAP analysis shows no signs of gliosis in the cortex or hippocampus of EEG recorded (Rec) neonatal mice compared to naïve (Naïve) controls; n = 8 Rec, n = 6 Naïve. Scale bar is 500μm.