CX3CL1 and CX3CR1 expression in murine gestational tissues after LPS injection.

(A) Gene expression of Cx3cl1 in the gestational tissues (placenta and amnion) of pregnant WT mice at 6 h after LPS or PBS injection at gd 15.5. (B) Detection of CX3CL1 protein in murine gestational tissues. Immunohistochemical analysis was performed using anti-CX3CL1 Abs in WT mice at gd 15.5. Representative results from 6 independent animals are shown here. Original magnification, x200. (C) Serum CX3CL1 concentrations in non-pregnant and pregnant WT mice. The serum was collected at the indicated time points of pregnancy. Serum CX3CL1 levels were measured as described in Materials and Methods. Statistical significance determined using Mann-Whitney’s U test, **P U test, **P < 0.01, vs. PBS treatment (n = 5–8 cases in each group). (F) Double-color immunofluorescence analysis for the identification of CX3CR1-expressing cells in the uterus of pregnant WT mice at 6 h after LPS injection. Representative results from six independent experiments are shown here. Signals were digitally merged (F4/80 and CX3CR1). Original magnification, ×400.