Additional file 2: of Identification of transcription factor genes involved in anthocyanin biosynthesis in carrot (Daucus carota L.) using RNA-Seq
- Miyako Kodama (5614823)
- Henrik Brinch-Pedersen (1759138)
- Shrikant Sharma (1819531)
- Inger Holme (3600365)
- Bjarne Joernsgaard (3905599)
- Tsaneta Dzhanfezova (3905605)
- Daniel Amby (3528977)
- Filipe Vieira (4908736)
- Shanlin Liu (2851853)
- M Gilbert (5947919)
Publication date
November 2018
DOIAbstract
Sequence and primer properties used to analyses 11 candidate genes and GAPDH (Additional file 2.1), PCR efficiencies calculated for primer pairs using 4 fold (based on mean Ct values observed for triplicates) dilution series of cDNA mix from all 30 samples (Pfaffl 2001; Additional file 2.2), relative expression ratio of 11 candidate genes in purple vs non-purple calli/tissue (Average of biological samples) calculated with following formula (Pfaffl 2001; Additional file 2.3); trimmed mean of M-values (TMM) of 11 candidate genes in purple and non-purple calli/tissue (Additional file 2.4). (XLSX 58 kb
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