A. Light microscopy and scanning electron microscopy (SEM) images of MSCs on three different scaffolds. Scale bar = 50μm. B. Proliferation assay for MSCs cultured in different scaffolds (n = 3). C. Cell cycle assay of MSCs after 5 days culture on collagen scaffolds (3D-Collagen), chitosan substrates (3D-Chitosan) and PLGA substrates (3D-PLGA). MSCs cultured on conditional 2D surfaces served as the control.</p
<p>Specific fluorescent staining of (A) NIH3T3, (B) C2C12 and (C) HeLa mammalian cells within the ce...
<p>Representative SEM images depicting the topology of surfaces coated with fibrillar (A) or non-fib...
A. Effects of 3D substrates on the proliferation of T lymphocytes. CFSE-labeled dividing cells after...
<p>A) Cell spreading was observed on PCL, PCL/HA, and PCL/col/HA scaffolds, but not on 100% collagen...
A. qRT-PCR for Oct4, Sox2 and Nanog of MSCs cultured in different substrates. MSCs cultured on condi...
<p>MSC were seeded in equal numbers (1000 cells/cm<sup>2</sup>) on tissue culture dishes which were ...
<p>(A) LLC1 cells cultured on 3P scaffold and 3P/chitosan composite scaffolds for 4 days. Top panel:...
<p>The click-iT EdU assay was performed on C2C12 myoblasts cultured for 3 days in proliferation medi...
<p>At day 1 (A) and day 3 (B) cells penetrated to scaffolds with different cells infiltration distan...
<p>(a) Long, spindle-shaped cells attached to the walls of the scaffold after 3 days (SEM×1000). (b)...
<p>Proliferation of differentiated BMSCs seeded on the oriented scaffold (filled squares) or the ran...
<p>(A) LLC1 cells (5×10<sup>3</sup>) were cultured on monolayer, PLGA and 3P scaffolds from day 1 to...
3-dimensional microcarrier (3D-MC) cell culture systems are often used for expansion of stem cells i...
<p>A) Calcein cell viability assay. A1) Fluorescence image of a cell-seeded scaffold after 3 days. G...
<p>The scaffolds were suspended in cell culture inserts as described in materials and method section...
<p>Specific fluorescent staining of (A) NIH3T3, (B) C2C12 and (C) HeLa mammalian cells within the ce...
<p>Representative SEM images depicting the topology of surfaces coated with fibrillar (A) or non-fib...
A. Effects of 3D substrates on the proliferation of T lymphocytes. CFSE-labeled dividing cells after...
<p>A) Cell spreading was observed on PCL, PCL/HA, and PCL/col/HA scaffolds, but not on 100% collagen...
A. qRT-PCR for Oct4, Sox2 and Nanog of MSCs cultured in different substrates. MSCs cultured on condi...
<p>MSC were seeded in equal numbers (1000 cells/cm<sup>2</sup>) on tissue culture dishes which were ...
<p>(A) LLC1 cells cultured on 3P scaffold and 3P/chitosan composite scaffolds for 4 days. Top panel:...
<p>The click-iT EdU assay was performed on C2C12 myoblasts cultured for 3 days in proliferation medi...
<p>At day 1 (A) and day 3 (B) cells penetrated to scaffolds with different cells infiltration distan...
<p>(a) Long, spindle-shaped cells attached to the walls of the scaffold after 3 days (SEM×1000). (b)...
<p>Proliferation of differentiated BMSCs seeded on the oriented scaffold (filled squares) or the ran...
<p>(A) LLC1 cells (5×10<sup>3</sup>) were cultured on monolayer, PLGA and 3P scaffolds from day 1 to...
3-dimensional microcarrier (3D-MC) cell culture systems are often used for expansion of stem cells i...
<p>A) Calcein cell viability assay. A1) Fluorescence image of a cell-seeded scaffold after 3 days. G...
<p>The scaffolds were suspended in cell culture inserts as described in materials and method section...
<p>Specific fluorescent staining of (A) NIH3T3, (B) C2C12 and (C) HeLa mammalian cells within the ce...
<p>Representative SEM images depicting the topology of surfaces coated with fibrillar (A) or non-fib...
A. Effects of 3D substrates on the proliferation of T lymphocytes. CFSE-labeled dividing cells after...