Effects of different stimuli on iNOS synthesis in THP-1 activated macrophages.

(A) Macrophages were activated with WT and SCV strains respectively for 0 h, 2 h, 24 h. Data are expressed as means ± SD and are representative of 3 independent experiments. K indicates negative control of untreated and unstimulated cells. Where indicated *p(B) IFN-γ primed cells were infected with bacterial strains for indicated time points post infection. Data are expressed as means ± SD and are representative of 3 independent experiments. K indicates negative control of untreated and unstimulated cells. IFN indicates macrophages pre-treated with IFN-γ. Where indicated *, **, ***p(C) cells were treated with WT or SCV in presence of rapamycin for 0 h, 2 h, 24 h post infection. iNOS synthesis was assessed by ELISA in the incubation medium. Data are expressed as means ± SD and are representative of 3 independent experiments. Data are expressed as means ± SD and are representative of 3 independent experiments. K indicates negative control of untreated and unstimulated cells. Rapa indicates macrophages treated with rapamycin. Where indicated *, **, ***p(D) Effect of priming with IFN-γ and rapamycin on iNOS concentration in relation to S. epidermidis WT and SCV. Data are expressed as means ± SD and are representative of 3 independent experiments. Where indicated #p<0.05 WT vs. IFN + WT; # # #p<0.05 IFN + WT vs. Rapa + WT; *p<0.05 SCV vs. IFN + SCV; ***p<0.05 IFN + SCV vs. Rapa + SCV.