Plaque size is maintained in C6/36 cells, but not in Vero cells.

ZIKV-FLRmos was plaqued on Vero cells and representative tiny (L1), small (L2), or medium (L3) plaques were plaque purified over three rounds of passage through C6/36 cells. Similarly, ZIKV-FLRmam was plaqued on Vero cells and representative tiny (L1), small (L2), medium (L3), large normal (L4) or large with a fried-egg phenotype (L5) plaques were plaque purified over three rounds of passage through Vero cells. Each passage of virus was titered on Vero cells. A representative of the final passage of each lineage is shown (A). The area of 30–50 plaques from each passage of each lineage derived from ZIKV-FLRmos (B) and ZIKV-FLRmam (C) was measured using ImageJ.