Overexpression of PAFAH2 represses PM-induced EC barrier dysfunction.

(A, B, C) Cells were transfected with plasmid encoding human PAFAH2 or control empty vector for 24 h followed by treatment with PM (20 μg/cm2, 4 hr). Cell lysates were analyzed by Western blotting to determine phospho-VE-cadherin (A), membrane bound VE-cadherin (B) and biotinylated VE-cadherin (C) levels. Detection of VE-cadherin in total cell lysates was used as normalization control. (D) Cells transfected with empty control vector or PAFAH2 overexpressing plasmid were treated with PM and TER was monitored over time. Data are expressed as mean ± S.D.; n = 4.