Additional file 1: of Targeting myeloid-inflamed tumor with anti-CSF-1R antibody expands CD137+ effector T-cells in the murine model of pancreatic cancer

Figure S1. PD-1 expression on CD4+ and CD8+ T-cells can increase with the addition of αCSF-1R to GVAX therapy and αPD-1. Liver metastatic KPC tumor-bearing mice were sacrificed on day 14 after receiving treatment, and flow cytometry analysis was performed on the isolated tumor infiltrating immune cells. PD-1 expression within the (A) CD45 + CD4+ T-cell and (C) CD45 + CD8+ T-cell populations. The number of (B) CD45 + CD4 + PD-1+ and (D) CD45 + CD8 + PD-1+ T-cells. N = 3 for each treatment group, and the cells from the mice from the same treatment group were pooled and measured in triplicates. * p < 0.05; ** p < 0.01; *** p < 0.001; NS, non-significant. (PNG 32 kb