Delayed oseltamivir plus sirolimus treatment reduced inflammatory cell infiltration.

(A) Histopathological examination of the inflammatory cell infiltration into the lungs of infected mice in different treatment groups at 5 dpi. Scale bar = 100 μm. (B) Lung inflammatory cell infiltration scores of infected mice in different treatment groups. (C) Representative immunohistochemical images of CD68 expression in the lungs of the infected mice in different treatment groups at 5 dpi. Scale bar = 200 μm, original magnification: ×100. (D) Quantitative analysis of CD68-positive cells in the lungs of infected mice in different treatment groups. (E) Relative MPO activity in the lung homogenates of infected mice in different treatment groups at 5 dpi. (F) Total cell counts and (G) the numbers of neutrophils, macrophages, and lymphocytes in BALF samples from different treatment groups at 5 and 7 dpi. Data are representative of two independent experiments and presented as mean ± SEM (n = 5 per group). *, **, and *** represent p < 0.05, 0.01, and 0.001, respectively.