Gene expression analysis of iron metabolism, oxidative stress and inflammation markers in bone tissue.

a) Iron metabolism: ferroportin (Slc40a1), ferritin heavy polypeptide 1 (Fth1) and transferrin receptor 1 (Tfrc). b) Oxidative stress: catalase (Cat). c) Inflammation response: Tumor necrosis factor (Tnf) and Interleukin 6 (Il6). Relative expression was obtained through ΔΔCt method and was normalized with Gapdh. Each group analyzed was composed by n = 6 animals for [C+I], [KO] and [KO+I], and by n = 5 for [C]. Statistical comparison was performed with T-test (Welch’s correction) between control and iron-enriched diets for both WT ([C] and [C+I]]) and Hfe-KO mice ([KO] and [KO+I])). Group deviations are expressed as standard error to the mean (SEM). Statistical significance: (*)-p<0.05; (**)-p<0.01; (***)-p<0.001.