Histological analysis.

A. Microscopic variables for the effective ablation. Measurement of fluoroscopic ablation length was based on the electrode marker and histological ablation length was based on the length of necrosis parallel to the bile duct in consecutive slides. Effective ablation was calculated as histological ablation length over fluoroscopic ablation length as a percentage. Maximum depth of ablation defined as necrosis between the basal lamina of the normal epithelium and the most deeply positioned necrotic inflammation. B. Microscopic image of the bile duct. Dotted line shows the margin of necrotic tissue and black arrow head shows malignant cell infiltration.