Histological analysis.

(A) Representative histological photographs at 28 days after surgery of the four groups [control without the enamel matrix derivative [EMD] and without the cement enamel [CE(-)]; control with EMD, CE(+); diabetes without EMD, DE(-); and diabetes with EMD, DE(+)]. In the mesio-distal section, the defects were filled with newly formed connective tissue, cementum, and bone. Newly formed bone was observed along the roots of surgical defects. Hematoxylin–eosin staining, magnification 10×. Scale bar: 500 μm. (B) Higher magnification of the interface of the original bone (OB), newly formed bone (NB), and newly formed cementum (NC) at the bottom level of the original defect (arrowheads). Hematoxylin–eosin staining, magnification 40×. Scale bar: 100 μm.