The effect of C-terminal RTP mutants on the accumulation of virus in different host plants.

(A) Genome organization of PLRV and a description of the readthrough deletion mutants used in this study. (B) Virus titer measured by DAS-ELISA at 5 wpi in three immature leaves collected from each of 15 hairy nightshade plants systemically infected with wild-type (WT) PLRV and the C-terminal mutants shown in panel A and a mutant (Mut-5670-TAA that had TAA stop codon inserted at nucleotide 5670 that would prevent the translation of the five amino acid domain. (C and D) Virus titer measured by DAS-ELISA at 5 wpi in three immature leaves collected from each of 15 N. benthamiana (C) and P. floridana (D) plants systemically infected with WT-PLRV or each of the three C-terminal mutants that defined the 5-aa motif. Letters above the bars in (B), (C), (D) indicate significant differences revealed by Dunn’s multiple comparisons test p<0.05.