Additional file 6: of Mechanical ventilation enhances extrapulmonary sepsis-induced lung injury: role of WISP1–αvβ5 integrin pathway in TLR4-mediated inflammation and injury

Figure S6. Integrin β5 upregulation by LPS is NF-κB dependent. Western blot for nuclear and cytoplasma NF-κB p65 (A) and immunostaining of NF-κB p65 (C) in peritoneal macrophages (PM) induced by LPS over time. Increase in integrin β5 protein levels induced by LPS at 4.5 h significantly decreased by inhibitor of NF-κB signaling, IKK-NBD (B). *P < 0.05; **P < 0.01; ***P < 0.001 (TIF 613 kb