Validation of up-regulated genes using Real-time PCR analysis.
- Seok-Jun Moon (5983010)
- Hee Jin Park (567147)
- Tae-Heon Kim (2013925)
- Ju-Won Kang (3520262)
- Ji-Yoon Lee (2191789)
- Jun-Hyun Cho (5983013)
- Jong-Hee Lee (242907)
- Dong-Soo Park (1765399)
- Myung-Ok Byun (5983016)
- Beom-Gi Kim (445351)
- Dongjin Shin (3488921)
Publication date
November 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
Total RNA was extracted from two-week old WT or OsTGA2 overexpressing transgenic plants treated with 2 mM SA and used for cDNA synthesis followed by Q-RT PCR to check expression levels of stress related genes: (A) AP2/EREBP129, (B) ERD1, (C) indole-3-acetate O-methyltransferase 1-like, jasmonate O-methyltransferase, (D) HSP70-HSP90 organizing protein 1, (E) DEAD-box ATP-dependent RNA helicase 12-like, (F) peptidyl-prolyl cis-trans isomerase FKBP65, (G) YSL13, (H) glycine-rich RNA-binding, (I) Conserved hypothetical protein (Os08g0351300), (J) Conserved hypothetical protein (Os08g0395700), (K) UDP-glucuronosyl/UDP-glucosyltransferase family protein, (L) auxin-induced protein 5NG4.</p
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