Evaluation of <i>EGFR</i> T790M detection limitation by PNA-MALDI-TOF MS.
- Kang-Yi Su (133405)
- Jeng-Sen Tseng (628945)
- Keng-Mao Liao (5983022)
- Tsung-Ying Yang (244469)
- Kun-Chieh Chen (244491)
- Kuo-Hsuan Hsu (628949)
- Pan-Chyr Yang (19231)
- Sung-Liang Yu (215636)
- Gee-Chen Chang (244418)
Publication date
November 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
Wild-type (pcDNA3.1-EGFRwild-type) and mutant (pcDNA3.1-EGFRL858R/T790M) EGFR expression plasmids were proportionally mixed to generate serial mutation ratio samples to test the limit of detection. MALDI-TOF MS without EGFR T790M PNA had a detection sensitivity approximating 1% both for L858R and T790M. T790M, but not L858R, had a 0.1% detection sensitivity when combined with PNA.</p
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