MOESM1 of Increased activated regulatory T cell subsets and aging Treg-like cells in multiple myeloma and monoclonal gammopathy of undetermined significance: a case control study

Additional file 1. The gating strategy of Treg subsets. Phenotype of Treg cell subsets: resting Treg cells, activated Treg cells and non-Treg cells. (A) PBMCs or BMMCs were gated on FSC, SSC and analyzed for lymphocytes. (B) Percentages of CD4+ T cells gated on CD4 and SSC. (C) Three subsets of CD4+ T cells are defined by the expression of CD45RA and FoxP3: CD45RA+FoxP3lo cells, CD45RA-FoxP3hi cells, CD45RA-FoxP3lo cells; Representative dot plots are shown for an untreated MM patient